Great Nanog Antibody from GeneTex for Western Blot

October 24, 2018

Department of Laboratory Medicine
MacKay Memorial Hospital
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

Nanog antibody [GT3312]

Catalog Number:

GTX627421

This antibody was successfully used to detect Nanog expression in iPSCs by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Induced pluripotent stem cell

Primary Incubation

1:1000 dilution overnight at 4°C in blocking reagent

Blocking Agent

5% non-fat dry milk in TBST

Secondary Incubation

1:1000 dilution 1 hr at 25°C in blocking reagent

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

This antibody perfectly detected Nanog expression by Western blot in iPSCs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

We will continue to use this antibody.

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