Sensitive GFP Antibody in Drosophila Brain that Can Recognize GRASP

October 18, 2018

Institute of Biotechnology
National Tsing Hua University
Ph.D. Student

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Company:

Invitrogen (ThermoFisher)

Product Name:

GFP Tag Monoclonal Antibody (3E6)

Catalog Number:

A11120

We use this antibody to label GFP signals after reconstituted from GRASP (GFP reconstitution across synaptic partner)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Drosophila Brain

Primary Incubation

1:100 4 degree overnight

Blocking Agent

5% normal goat serum in 0.3%PBST

Secondary Incubation

1:200 in RT for 4hrs

Tertiary Incubation

N/A

Detection

Alexa-488 immunofluorescence

Results Summary

We express GRASP in fly's eye system DM8 and TM5c neurons and using this antibody to detect reconstituted GFP signals. This antibody is much more sensitive and stronger than another item we've used before. Green: reconstituted GFP; Magenta: DM8 neurons; Red:TM5c neurons.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitive, clean and brighter

The Bad

Little background

The Bottom Line

This antibody is good to use in Drosophila Brain to detect reconstituted GFP signals through GRASP.

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