Good LC3 Antibody for Western Blot and Immunofluorescence

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signalling Technology

Product Name:

LC3 Antibody

Catalog Number:

2775S

Used the LC3 antibody to carry out Autophagy studies in the cells and as well as immunofluorescence studies. In summary, we are able to see how the protein expression level changes in respond to different type of pathogen strains.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Gastric cancer cell line AGS cell lysate

Primary Incubation

1:1000 dilution overnight at 4 deg

Blocking Agent

1% Milk

Secondary Incubation

IRDye® 800CW Goat anti-Rabbit IgG (H + L), 0.1 mg

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

The results give a very sharp and clear band as seen in the picture and we are able to see how the protein expression level changes in respond to different type of pathogen strains.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to administer and we are able to detect both LC3-I and LC3-II

The Bad

N/A

The Bottom Line

Very good antibody from the company and is highly recommended for those who are carrying out such studies.

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