One World Lab
MESDC1 Antibody - N-terminal region
ARP67656_P050
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I am interested in studying the molecular mechanism of hedgehog signal transduction in vertebrate cells. I have performed a CRISPR/Cas9 genetic screen to uncover new regulators of hedgehog signaling pathway. One of the negative regulators identified in my screens was MESDC1. In this experiment, I had knocked out MESDC1 in NIH-3T3 cells using lentiCRISPR v2 strategy. The result is a pooled knockout cell line for MESDC1. MESDC1 antibody from One World Lab picked up a band at ~38 kDa. The predicted size is 38 kDa.
Western Blot
NIH-3T3 whole-cell extract
1:500 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% milk in TBS-T
1:10,000 dilution of donkey anti-rabbit IgG+HRP; 1 hr at room temperature
None
ECL
Mesdc1 antibody picks up a band at 38 kDa (expected size). The antibody also picks up three more non-specific bands.
N/A
Picks up a right-sized band
A few non-specific bands
Good for western blotting