Abcam
Anti-beta Actin antibody [AC-15] (HRP)
ab49900
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Our aim was to determine changes in methylation pattern of H3K9me3 by changing Setdb1 expression in PC3 cells. For this study, beta-actin was used as one of the controls to determine total protein loaded per sample.
Western Blot
Human cell lines PC3 lysates
Overnight, 4 degree C, 1:5000
5% milk
No secondary required
N/A
Hrp-dab reaction
Changes in methylation pattern of H3K9me3 were not observed by changing the expression of Setdb1. Vinculin was used as a loading control.
Highly specific, no cross reactivity
None
Good control antibody for western blots. Doesn't need a secondary Ab so its fast.