MilliporeSigma
Monoclonal Anti-ß-Actin antibody
A5441
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The monoclonal anti-beta-actin was used to detect beta-actin as loading control in the western blot method. This primary antibody was recommended by colleagues.
Western Blot
Protein extract
1:10,000
5% non-fat milk
Anti-mouse secondary antibody at 1:20,000
None
Chemiluminescence detection method
Beta-actin was easily detected. 20 seconds of exposure was sufficient to detect this protein.
N/A
Highly sensitive, reliable, and easy to use.
The antibody can be reused multiple times (3X) when stored in the fridge.