BioTek Synergy H1 Hybrid Multi-Mode Microplate Reader

October 15, 2018

Stanford University
Biochemistry
Senior Research Scientist

Overall

Performance

Ease-of-Optimization

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Company:

BioTek

Product Name:

Synergy H1 Hybrid Multi-Mode Microplate Reader

Catalog Number:

8041000

I'm interested in understanding the molecular mechanism of hedgehog signal transduction. Some of the experiments used for measuring the pathway activity include luciferase assays and protein estimations (BCA or Bradford). This product was extensively tested during demo phase and then purchased by our lab with the dual injection port module for luciferase assays.

Experimental Design and Results Summary

Application

Luciferase assay

Protocol Overview

Cells were transfected with the Firefly luciferase and Renilla luciferase constructs. After specific treatments, cell lysates were prepared and the Firefly and Renilla luciferase activities were measured in this system by using the dual injection port module (well-by-well).

Tips

Follow the user instructions.

Features Summary

I have been using it for luciferase assays and protein estimations, but this machine is routinely used by other lab members for various spectroscopy applications.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

We had purchased both monochromator-based optics and filter-based optics for flexibility and sensitivity. We also opted for top and bottom fluorescence intensity measurement. This is one of our most widely used instruments in the lab and has had no issues for the past 4 years.

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