Reverse Transcribe Total RNA

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

TaqMan™ Reverse Transcription Reagents

Catalog Number:

8080234

The Taqman reverse transcription kit was used to reverse transcribe total RNA extracted from cells. The kit was recommended by colleagues.

Experimental Design and Results Summary

Application

Reverse Transcription

Starting Material

Total RNA

Protocol Overview

Follow as per manufacturer's protocol.

Tips

Perform DNA digest prior to reverse transcription when total RNA is extracted from tissue samples.

Results Summary

Total RNA were successfully reverse transcribed. This was assessed by real-time PCR.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy-to-use one-step reverse transcription method.

The Bad

Expensive

The Bottom Line

This kit is useful when the protocol requires 50:50 oligo dT and random hexamer primer mix.

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