Good Anti-Human CD19 PE-Cy7

October 11, 2018

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

eBioscience

Product Name:

CD19 Monoclonal Antibody (HIB19), PE-Cyanine7

Catalog Number:

25-0199-42

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+CD20+) in isolated PBMCs from peripheral blood of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Isolated PBMCs from healthy donor peripheral blood

Primary Incubation

anti-CD19 PE-Cy7, 15min in the dark

Blocking Agent

Fc block, 10 min RT

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytometer

Results Summary

We found 40% of the live lymphocytes CD3-CD14-CD16- are B cells expressing CD19 and CD20.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works

The Bad

There is some unspecific staining

The Bottom Line

It should be used together with another marker of B cells, but it works.

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