Luminex
Guava Cell Cycle Reagent
4500-0220
Info:Supplier Page
We wanted to examine changes in cell cycle when we knocked out a gene of interest in the presence and absence of radiation. We used this kit with flow cytometry to determine cell cycle distribution.
Cell cycle via flow cytometry
Cal27 Cell Line
Cells can be kept in PBS after ethanol fixation until staining is performed.
We saw a G2/M arrest in both the parental and knockout cell lines when we treated with 4Gy of radiation.
N/A
A very easy kit to use with clear signal!
Single readout (no additional markers for apoptosis, etc.)
This kit was easy to use and gave us clear, understandable results.