Miltenyi Biotec
Plexin-B2 Antibody, anti-mouse, Vio ® Bright FITC , REAfinity™
130-106-410
REA445
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are looking for markers of PC.
Flow Cytometry
Mouse splenocytes
Surface staining with anti-plexinB2 Vio Bright FITC )(1/50), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). Plexin-B2 is suppose to be expressed by subsets of B cells. Here we see a shift, but not a clear staining.
There is a signal.
The signal is not reliable.
The use of this antibody is difficult.