Cell Signaling Technology
NF-κB p65 (D14E12) XP® Rabbit mAb
8242
D14E12
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This antibody was used to detect endogenous levels of total NF-KB p65 protein from human cell lysates (25 ug/mL protein loading) through western blot analysis.
Western Blot
Human endothelial cells
1:1000, overnight@4ºC
5% BSA/TBS
Anti-rabbit IgG HRP (#HAF008, R&D systems), 1:5000
N/A
Chemiluminescent
We used this antibody to normalize phospho-p65 protein levels. The antibody is really sensitive and provides robust results with low exposures. It can be reused at least twice when diluted in BSA/TBS and kept at 4ºC.
Consistent, sensitive, robust
None
It can be reused at least twice when diluted in BSA/TBS.