Great TCR Beta Chain Antibody

Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse TCR β chain Antibody

Catalog Number:

109207

Clone Number:

H57-597

Our group focuses on TCR gene transfer. This antibody is commonly used in our work to evaluate the transduction efficiency of the TCRs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

1:100, 20 min RT

Blocking Agent

N/a

Secondary Incubation

N/a

Tertiary Incubation

N/a

Detection

BD LSR II

Results Summary

The introduction of the TCR into PBMCs via retroviral transduction was evaluated by staining the mTCRb that's inclulded in our construct.

DOI or PMID #

Additional Notes

Image Gallery

Summary

The Good

Works well

The Bad

None

The Bottom Line

Excellent antibody, staining is consistent between new vials and the amount required is low.

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