Very Good Anti-Human CD138

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BD Horizon™ BUV737 Mouse Anti-Human CD138

Catalog Number:

564393

Clone Number:

MI15

Info:Supplier Page 

Browse Similar Products: CD138 Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment, we are looking for markers of PC, so we stain them using anti-CD138.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human BM

Primary Incubation

Surface staining with CD138 BUV737, 20 min at 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). We stained BMPC using CD138 and CD38, two well established markers of PCs.

DOI or PMID #

35464469

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal.

The Bad

It is expensive.

The Bottom Line

Antibody has great signal!

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