Thermo Fisher Scientific
IL-21 Monoclonal Antibody (mhalx21), PE, eBioscience™
12-7213-82
mhalx21
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I used this antibody to test stimulated T cells isolated from a knockout mouse model. The antibody gave a clear signal.
Flow Cytometry
Mouse blood
1:100 for 30 min @ 4C
Fc block
N/A
Flow cytometer
After stimulation and treatment with a golgi inhibitor, cells were fixed and permeabilized and stained with the IL-21 antibody. The results showed a slight increase in IL-21 production between unstimulated and stimulated, with minimal background staining.
Good staining.
Some background.
Good antibody but somewhat expensive.