Good Anti-Human CD39 Antibody

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-human CD39 Antibody

Catalog Number:

328213

Clone Number:

A1

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment we are looking for markers of PC.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Isolated PBMCs from HD peripheral blood

Primary Incubation

surface staining with CD139 BV421, 20 min at 4C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry with a violet laser

Results Summary

In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). We found that CD39 is higher expressed by PC (CD20-) in comparison with B cells (CD20+).

DOI or PMID #

35464469

Additional Notes

N/A

Image Gallery

Summary

The Good

It is bright.

The Bad

N/A

The Bottom Line

It works well. Is a good option to have.

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