Cleaved Caspase 3 (Asp175) Antibody for Western Blot

July 22, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Cleaved caspase 3 (Asp175) antibody

Catalog Number:

9661

Clone Number:

N/A

This antibody was used to detect cleaved caspase-3 levels in western blot analysis. Protein loading: 25 ug/mL.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:500, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-rabbit IgG HRP (#HAF008, R&D systems), 1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

The antibody does not always detect cleaved caspase-3 levels or sometimes only one of the bands appears. It is necessary high exposures for detection. It also required some optimizations to have a signal.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Dilution in BSA/TBS can be reused at least 3x.

The Bad

Weak signal.

The Bottom Line

Results not always are consistent even with the same samples.

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