Great Kit for Protein Quantification

July 25, 2022

BlueskyReddit
Yale University
Department of Pharmacology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Thermo Scientific

Product Name:

Pierce BCA Protein Assay

Catalog Number:

23225

We use this kit routinely to quantify the amount of protein in cell lysates to be used in western blots. The kit is easy to use and accurately quantifies protein.

Experimental Design and Results Summary

Application

Protein quantification

Starting Material

Monolayer cell lines

Tips

We tend to run out of "Reagent A" before we run out of "Reagent B".

Results Summary

This product allows us to quantify the amount of protein in our cell lysates to be used in western blots. Our loading controls indicate that our quantification is accurate.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

The kit is easy to use and accurately quantifies protein.

The Bad

The kit requires a 30 minute incubation before the protein absorbance can be read.

The Bottom Line

We use this kit to routinely quantify our cell lysates to use in western blots and it works really well!

Join the discussion