Biotin anti-mouse CD229 (Ly-9) Antibody
122903
Ly9ab3
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment, we are looking for markers of PC.
Flow Cytometry
WT mouse spleen
Surface staining with CD229 biotin, 20 min at 4C
Fc block (2.4G2 clone)
SA-PE or any other SA
N/A
Flow cytometer A5 Symphony BD
In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). We found that CD229 is higher expressed by PC in comparison with B cells.
35464469
It works.
It is coupled with biotin.
It is a versatile option to stain mouse CD229.