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Date Published
Anti-SLC19A1 Picoband Antibody Didn't Work for WB
(1)
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BosterBio
December 19, 2022
I have tried this antibody for 4 times with different tissue and cell lysate from different species. There is no clear band observed at the ...
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Good Antibody Against CXCR3 (CD183)
(1)
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BioLegend
December 16, 2022
In different healthy and pathological contexts, CD4 and CD8 T cells differentiate into a range of specialized effector and regulatory ...
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Good Product for Efficient Lentivirus Transduction
(1)
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December 16, 2022
We used this reagent to increase the lentiviral transduction of COS7 cells. TransDuX MAX application increased the efficiency of lentiviral-...
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Great Reagent for PNH
(1)
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CEDARLANE
December 16, 2022
In our lab, we test for paroxysmal nocturnal hemoglobinuria (PNH). Although there are antibodies for this application, the addition of FLAER...
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Excellent Western Blot Stripping Buffer
(1)
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Thermo Fisher Scientific
December 16, 2022
We have been using this buffer to strip the bands for Western Blot. This buffer works great and can safely and effectively remove primary ...
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Easy Bcl-2 Detection for Western Blot
(1)
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Cell Signaling Technology
December 16, 2022
This mouse monoclonal Bcl-2 antibody from Cell singaling is our standard antibody for the detection of endogenous and overexpressed Bcl-2 in...
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LC3 Antibody Works Well
(1)
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Cell Signaling Technology
December 16, 2022
25mg mouse liver lysate was applied into 10% SDS-page and incubated with LC3 antibody (1:1,000, in 5% BSA) overnight.
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Western Blot on Fibroblast
(1)
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Cell Signaling Technology
December 16, 2022
I used this antibody for the WB. I stimulated the MRC-5 with TGF-b for 24 h. I incubated the primary antibody overnight at 4 °C (1:1000) and...
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Great p44/42 MAPK (Erk1/2) Antibody for Western Blotting
(1)
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Cell Signaling Technology
December 15, 2022
Very strong antibody for western blotting total Erk in MCF10a cell lysates. Strong signal so only small amount of antibody needed.
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Good Option to Stain for Ki67
(1)
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BioLegend
December 15, 2022
In different healthy and pathological contexts, CD4 and CD8 T cells differentiate into a range of specialized effector and regulatory ...
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Good CD57 MicroBeads for Magnetic Isolation
(1)
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Miltenyi Biotec
December 15, 2022
We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely require ...
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Good Option to Stain for CD62L
(1)
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BioLegend
December 15, 2022
In different healthy and pathological contexts, CD4 and CD8 T cells differentiate into a range of specialized effector and regulatory ...
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Reliable IL-8 ELISA kit
(1)
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BioLegend
December 15, 2022
RAW 264.7 a mouse macrophage cell line as an in vitro cell culture model. Following treatments, collected conditioned media for measuring ...
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Surprisingly Reproducible Ras G-LISA Activation Assay Kit
(1)
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December 15, 2022
This kit was used to assay Ras activity in isolated mouse neutrophils. Nice results, despite poor reputation of GLISA.
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T Cell Staining for Live Imaging
(1)
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Biotium, Inc.
December 14, 2022
Primary T cells were stained with MemBrite Fix Cell Surface Stain and cultured on unstained primary endothelial cells to analyze T cell ...
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Great Anti-5-Methylcytosine Monoclonal Antibody
(1)
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December 14, 2022
We were interested in studying the role of DNA methylation in CD4 T-cells. A variety of antibodies where used to measure different DNA ...
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Excellent 5-Carboxylcytosine Antibody
(1)
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December 14, 2022
We were interested in studying the role of DNA methylation in CD4 T-cells. A variety of antibodies where used to measure different DNA ...
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CD15 Antibody for Flow Cytometry Staining of MDSC
(1)
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BioLegend
December 14, 2022
We used this antibody in a panel for classification of MDSC isolated from whole blood by Ficoll density gradient centrifugation. Stains ...
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Reliable Vinculin for WB
(1)
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Abcam
December 14, 2022
I used the anti-vinculin antibody at a dilution of 1:2000 as a loading control for western blot. I used 20 ug protein/lane of mouse brain ...
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Highly Recommend Human Inflammation Array Q3
(1)
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RayBiotech
December 14, 2022
I am profiling the cytokine in COVID-19 patients based on the polymorphism of several genes. My goal to use this array kit was to study the...
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Excellent CD57 Antibody
(1)
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BioLegend
December 13, 2022
We are an immunotherapy focused group with an interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely isolate ...
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Western Blot on Mouse Lung Tissue
(1)
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Abcam
December 13, 2022
I used this antibody on mouse lung tissue. Even if it's recommended to use for human cells I was able to detect the protein. The protein ...
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Great Mounting Medium for Immunostaining
(1)
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Fisher Scientific
December 13, 2022
We have been using this mounting medium for immunostaining for over 7 years. It worked great for protecting the signals and enhancing FITC ...
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Excellent Collagenase Type 2
(1)
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Worthington Biochemical Corporation
December 13, 2022
We have used several bottles of this Type 2 collagenase for dissociation of tissues. Type 2 collagenase contains higher relative levels of ...
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Good Kit for Myelinated Cell Removal
(1)
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Miltenyi Biotec
December 13, 2022
We used this kit to remove myelinated cells from mouse brain homogenate and enrich for endothelial cells. The protocol was fast and worked ...
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Good Anti-TCF1 Antibody
(1)
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BD Biosciences
December 13, 2022
In different healthy and pathological contexts, CD4 and CD8 T cells differentiate into a range of specialized effector and regulatory ...
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Great Antibody for Immunofluorescence
(1)
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Abcam
December 12, 2022
We used this antibody to analyze CXCL10 (IP10) expression from primary mouse brain endothelial cells in response to Interferon-gamma. ...
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Rodent Specific Antibody for Spliced XBP1
(1)
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Cell Signaling Technology
December 12, 2022
One of the best antibodies for detecting the expression of spliced XBP1 in mouse and rat samples. Highly specific!
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Eppendorf™ Centrifuge 5425
(1)
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Fisher Scientific
December 12, 2022
The Eppendorf™ Centrifuge 5425 replaced the old centrifuge in our lab that was an essential part of running PCR to genotype our colony of KO...
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Great Kit for Cloning Work
(1)
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Promega
December 12, 2022
This kit is an easy to use system to help users to clone PCR products generated by certain thermostable polymerases.
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Great Caveolin 1 Polyclonal Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
December 12, 2022
We used this antibody for immunostaining of primary brain endothelial cells from WT and Cav-1 KO mice. Staining was bright with little to no...
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CD41 Antibody Works Perfectly
(1)
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BioLegend
December 12, 2022
Biolegend antibodies are excellent for use in platelet staining since they are available in various sizes and fluorophore labels. We use ...
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Useful Kit for Measuring Cell Proliferation using Flow Cytometry
(1)
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December 12, 2022
This kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.
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Transduction with Less Toxicity
(1)
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Thermo Fisher Scientific
December 09, 2022
We use Lipofectamine 3000 reagent to transfect 293 cells for lentivirus production. This reagent is very gentle and allows us to lower the ...
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Excellent MAP2 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
December 09, 2022
The specific goal of this experiment was to evaluate MAP2 expression and dendrites in neurodegenerative diseases. This antibody worked very ...
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Great Kit for Labeling Mitochondria in Cells
(1)
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Thermo Fisher Scientific
December 09, 2022
The kit is a green-fluorescent mitochondrial stain which appears to localize to mitochondria regardless of mitochondrial membrane potential.
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XBP-1s (D2C1F) Rabbit mAb Lives Up to the CST Name
(1)
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Cell Signaling Technology
December 09, 2022
Used this antibody for both immunoblotting and in human platelet and tissue samples. Easy to use and works as recommended and produces clear...
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Fast Kit to Isolate T Cells from PBMC
(1)
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Miltenyi Biotec
December 09, 2022
In our lab, we use Pan T Cell Isolation Kit to quickly isolate T cells from human peripheral blood mononuclear cells (PBMCs).
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Good Poly(vinyl alcohol) for Electrospinning
(1)
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MilliporeSigma
December 08, 2022
I use PVA primarily for the production of a nanofibrous membrane. The nanofibrous membrane is prepared using the electrospinning technique ...
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CD41 Antibody Works Better than Expected
(1)
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BioLegend
December 08, 2022
Used this antibody as a stain for platelets. Although recommended for flow cytometry, also works very well in immunofluorescence experiments...
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Activation and Expansion of Human T Cells
(1)
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December 08, 2022
In our cell therapy research we isolate T cells and we use the reagent CD3/CD28/CD2 T Cell Activator together with IL-2 to activate and ...
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Acetonitrile HPLC-Grade
(1)
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Fisher Scientific
December 08, 2022
Acetonitrile is fundamental for running HPLC methods. Isocratic and gradient methods require the use of Acetonitrile in most cases. Thanks ...
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Excellent Anti-GRP78 BiP Antibody
(1)
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Abcam
December 08, 2022
Used this antibody to investigate the expression of GRP78 in the human and mice platelets following induction of ER stress.
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Prep-Kit Does the Job
(1)
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December 08, 2022
The work of our group is largely based on vectors and plasmids we use for transfections in human cell lines. To prepare our plasmids from ...
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Great Accutase
(1)
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Corning Life Sciences
December 08, 2022
Accutase is a cell detachment reagent very effective for different cell lines especially for the most delicate one. It is considered a mild-...
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ChIP-qPCR Kit with Sonication
(1)
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December 07, 2022
Generally, you can get your ChIP-qPCR results in 3 days: Day 1-2: perform the ChIP step; Day 3: qPCR analysis. A little reminder: with this ...
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Perilipin Antibody Working Great
(1)
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December 07, 2022
Antibody working great and accurate specificity. We look into Lipid droplets and the expression is nicely appeared on the Lipid droplets. ...
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Acetic Acid for HPLC Analysis
(1)
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Fisher BioReagents
December 07, 2022
I usually use Acetic acid for the development of HPLC methods. In particular I use acetic acid for acidifying the mobile phase i need for ...
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Good Beads for Magnetic Isolation
(1)
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Miltenyi Biotec
December 07, 2022
We are an immunotherapy focused group, with interest in CD8 T-cells and their TCRs. For our experimental purposes, we routinely require ...
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Easy Way to Remove CD19+ Cells from PBMC
(1)
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Miltenyi Biotec
December 07, 2022
In our experiment, we used CD19 microbeads to isolate CD19+ cells from human PBMCs (Peripheral blood mononuclear cells). We did use them to ...
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Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
Pipettor
Plate Reader
Precast Gels
Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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