Great T Cell Antibody for Flow Cytometry

Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

Overall

Quality of Results

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Company:

BioLegend

Product Name:

PE/Cyanine7 anti-mouse CD3 Antibody

Catalog Number:

100219

Clone Number:

17A2

Info:Supplier Page View Product Specs

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T cell infiltration into the brain during respiratory infection in mice was measured by staining for CD3 and analyzed by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse primary splenocytes and brain homogenate

Primary Incubation

1:200, 30 minutes on ice

Blocking Agent

TruStain FcX™ (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytoflex S flow cytometer (Beckman Coulter)

Results Summary

T cells in spleen and brain tissues were clearly labeled and easily identifiable by flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Low concentration needed, bright staining

The Bad

PE Cy7 fluorophore has significant spectral overlap with PE and APC fluorophores.

The Bottom Line

This antibody works very well to clearly label T cells for flow cytometry and is offered in many different conjugated forms from the supplier.

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