Recommended RAS Antibody from CST for Western Blot

January 20, 2023

Houston Methodist Research Institute

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Company:

Cell Signaling Technology

Product Name:

Ras Antibody

Catalog Number:

3965S

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Epithelial cells were seeded in 10 % FBS F-12 k. The day after the medium was replaced with 0.5% FBS and stimulated with PDGF (10 ng/ml) for 48 hours. The membrane was incubated overnight at 4 °C in TBST

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Epithelial cells were seeded in 10 % FBS F-12 k. The day after the medium was replaced with 0.5% FBS and stimulated with PDGF (10 ng/ml) for 48 hours. The membrane was incubated overnight at 4 °C in TBST

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Recommended.

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