Millipore Sigma
Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal
G1546-100UL
N/A
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We used this antibody to detect GFP tags in western blot applications. The antibody is not highly specific and works well at the suggested concentration.
Western Blot
Mammalian cell lysate
1:100 mouse anti-GFP primary antibody, 1% BSA in PBST
5% BSA in TBST
Goat anti-mouse IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT
ECL
Reasonably good antibody to detect GFP tags in transfected proteins through western blot. Works well at the suggested dilution. Easy to optimize. Specificity is not that great. Overall it's a good antibody.
Ease of optimization.
Not very specific, expensive.
It's a good-quality antibody. Not very specific. Slightly expensive.