Biolegend
PerCP/Cyanine5.5 anti-human TNF-α Antibody
502926
MAb11
Info:Supplier Page View Product Specs
Browse Similar Products: TNFalpha Antibodies
Our research aims to understand how some CD4 T cells become autoreactive in melanoma patients treated with check point inhibitors.In this experiment, we tested the expression of the pro-inflammatory cytokine TNF-a in CD4 T cells with an anti-TNF-a PerCP Cy5.5.
Flow Cytometry
Unstimulated and stimulated healthy donor's PBMCs
Washed twice in PBS and incubated with L/D fixable aqua dye 20 min at 4°C, then washed twice in PBS.
None
Surface staining with anti-CD4 PE dazzle594, CD3 BUV395, CD45RO BUV737 etc., 30' at 4°C, in the dark, in BD Horizon™ Brilliant Stain Buffer.
Cells were fixed and permeabilized with commercial kit (FoxpP3 transcription factor kit, eBioscience) for 30 min at 4°C with fixative, 15 min at 4°C with permeabilization buffer, and stained with anti-TNF-a PerCP Cy5.5 at 1/75 in permeabilization buffer.
Blue laser on Symphony cytometer
TNF-a-producing CD4+ T cells are readily detected. The staining decreases progressively in serial dilution of the antibody, but for some applications (high expressing leukicytes), it can be used at higher dilution than 1/75.
N/A
Easy to use, straight forward.
Needs to be carefully titrated, depending on application.
Very good option.