Reliable and Strong CD56 for Human NK Cells

LP2M
University of Cote d'Azur (UCA)
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE Anti-human CD56

Catalog Number:

362508

Clone Number:

5.1H11

Info:Supplier Page View Product Specs

Browse Similar Products: CD56 Antibodies

We work on NK subpopulations of the human immunosystem. We use this antibody in coupled with different fluorophores to identify the whole Natural Killer population and this PE coupled antibody gives a strong and precise staining of the cell of interest.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100 per 200-500k cells in 100ul, at 4°C for 20 mins

Blocking Agent

PBS + FCS 2%

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS Aria II

Results Summary

Very good antibody for FACS analysis on human cells. It works well in both purified population or whole isolated PBMCS, with a clear separation between the positive and negative populations.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strength of the specificity of the signal.

The Bad

None

The Bottom Line

The strong signal of this PE coupled antibody allows easy multiplexing with other antibodies to identify subclasses of human NK cells.

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