Great Thy1.1 Antibody for Flow Cytometry

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Catalog Number:

202529

Clone Number:

OX-7

The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system– the mammalian defense to homeostatic alteration. Ketogenic diets (KDs) are extremely high in fat and very low in carbohydrates and have been shown to dramatically rewire whole-body metabolism. The KD-induced metabolic adaptation improves many physiological processes including aging, fatty acid utilization, and glucose homeostasis. However, the effects of KDs on host immunity remain less explored1–3. Thus, the overall objective of my PhD studies will be to elucidate how KDs affect the immune response in adaptive and maladaptive contexts.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T cells

Primary Incubation

1:100

Blocking Agent

3%BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

A strong and clear signal of Thy1.1 on mouse CD4 T cells detected by flow cytometry after gating out dead cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable results.

The Bad

N/A

The Bottom Line

Good strong signal. Recommended for flow analysis and sorting.

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