Cell Signaling Technology
Caveolin-1 Rabbit mAb AF647
31411S
D46G3
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Changes in Caveolin-1 expression brain endothelial cells in response to respiratory infection was measured by flow cytometry.
Flow Cytometry
Primary mouse brain endothelial cells
1:100, 30 minutes on ice
TruStain FcX PLUS (anti-mouse CD16/32) Antibody
N/A
Cytoflex S Flow Cytometer (Beckman Coulter). APC channel
Caveolin-1 staining, as detected by flow cytometry, was bright and expression was elevated in the majority of mouse brain endothelial cells.
Cells permeabilized with FoxP3 Fix/Perm (Biolegend)
Very bright staining.
Expensive antibody.
This antibody works very well for flow cytometry staining. It is expensive, but we haven't found a less expensive option that works as well.