Excellent Antibody to Detect Collagen I

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-Collagen I antibody

Catalog Number:

ab34710

Clone Number:

N/A

We used this antibody to detect Collagen I levels in mammalian cell lysates. The antibody worked really well. The antibody is very specific. Highly recommended.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mammalian cell line whole cell lysate

Primary Incubation

1:1000, overnight incubation at 4 degree Celsius

Blocking Agent

5% BSA in TBST

Secondary Incubation

Goat anti-rabbit IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT

Tertiary Incubation

N/A

Detection

ECL

Results Summary

The antibody worked well at the suggested dilution. We observed a bright bright collagen I band at the expected size with minimal background. Results are highly consistent.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very specific.

The Bad

Expensive

The Bottom Line

A must-have antibody for Collagen I detection studies.

Share your experience with other scientists. Write a Review! »

Join the discussion