Turtle CREB Antibody for chromatin immunoprecipitation

DIVISION OF BASIC BIOMEDICAL SCIENCES, LEE MED BUILDING
UNIVERSITY OF SOUTH DAKOTA
POST-DOCTORAL FELLOW

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

CREB (48H2)

Catalog Number:

9197s

Info: View Product View Product Specs

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We are investigating the regulatory mechanisms controlling BDNF expression in an in vitro model of eyeblink classical conditioning, we analyzed the transcription factors and other signaling proteins that control BDNF gene expression in the pond turtle, Trachemys scripta elegans (tBDNF). To understand the mechanisms of epigenetic regulation of the tBDNF gene, we performed ChIP assay for CREB during classical conditioning.We got some good references for CREB to use the ChIP assay. We always have problem in selecting the antibodies for our model. We tried this antibody in our turtle system to analyze binding of CREB by ChIP analysis, it works.

Experimental Design and Results Summary

Applications

ChIP

Sample

Turtle brain stem

Primary Incubation

1:50

Blocking Agent

Salmon sperm DNA and Protein agarose A beads

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

ChIP-Real--time PCR was done. The results were good.

Additional Notes

None

Related Categories

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Summary

The Good

Concentrated antibody

The Bad

I think more glycerol needed.

The Bottom Line

It works very fine. I loved it. Cell singling rocks

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