Novus
TLR2
NB100-56573
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This antibody was used to assess inflammation in adipose tissue, as toll like receptor 2 is a pattern recognition receptor involved in activating inflammatory signaling cascades.
Western Blot
Mouse adipose tissue
1:1000 in 3% BSA in TBS-T overnight at 4C
3% BSA in TBS-T for 30 minutes at room temperature
1:5000 (sc-2317) in 5% Milk/TBS-T
None
GE Healthcare RPN2236 ECL Prime Western Blotting Detection Reagent, UVP 3 minute exposure
The antibody worked excellently in Western blot analysis.
Strong signal with small amount of protein
Very minimal background