Abcam
Anti-histone H3 (acetyl K27) antibody
ab4729
Info: View Product View Product Specs
Browse Similar Products: Histone H3 (acetyl K27) Antibodies
This product was used to determine if changes in this histone modification are associated with endothelial to mesenchymal transition. This antibody was recommended by a coworker.
Western Blot
Chromatin isolated from untreated and TGFb2 treated HUVECs
1:3000 dilution in 5% skim milk in TBS-t overnight at 4 degrees
5% skim milk in TBS-t for 1 hour at room temperature
1:10,000 dilution of anti-rabbit HRP conjugated secondary (thermo scientific Cat#31460) in 5% skim milk in TBS-t for 2 hours at room temperature
NA
Enhanced chemiluminescence
This histone modification was detected at 15kDa as a single band, and was decreased in HUVECs after treatment with TGFb2. Exposure time of 10 seconds with film.
None
Single band, very clean Western
Would highly recommend this antibody.