Brilliant Violet 605™ anti-mouse TNF-alpha Antibody

January 12, 2015

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-mouse TNF-α Antibody

Catalog Number:

506329

CD4 T cells were purified from the mouse spleen, TNF-alpha production was detected in the memory T cells by intracellular cytokine staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:100 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

This Ab can be used to detect mouse TNF-alpha production in CD4 memory T cells at low concentration.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy application

The Bad

None

The Bottom Line

Easy handling.

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