Bethyl Laboratories
Phospho SMC1 (S966) Antibody
A300-050A
Info: View Product View Product Specs
Browse Similar Products: SMC1 (S966) Antibodies
SMC1 is a subunit of cohesin complex that is required for chromosome maintenance, transcriptional regulation, and DNA repair. Phosphorylation of SMC1 is important for DNA damage-induced checkpoint activation.
Western Blot
HeLa human cancer cell line
Dilution 1:1000 in 5% non-fat milk/TBS-Tween20. Two hour incubation at room temperature.
5% non-fat milk/TBS-Tween20
HRP-conjugated anti-rabbit IgG
NA
Bio-Rad ChemiDoc imager
The UV-induced SMC1 phosphorylation was significantly increased compared to the undamaged sample. Total protein of SMC1 was used as loading control.
None
Very sensitive and specific
This is a very good antibody and can be used as a readout of the activation of upstream damage response kinases.