The Good
It works in many tissues and cell lines.
The Bad
Has to use ECL for best results, does not work well on LiCoR
The Bottom Line
A great antibody for biochemistry and microscopy - highly recommended for everyone new to the autophagy field and needs to check for metabolic state of the analysed cells. See description for more information about how to read the autophagy flux assays and immunofluorescence signals. Good luck!