Abcam
Anti-Histone H3 (mono methyl K4) antibody
ab8895
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Browse Similar Products: Histone H3 Western blot Antibodies
This product was used to determine if changes in H3H4me1 are associated with endothelial-mesenchymal-transition. This antibody was recommended to me by a co-worker.
Western Blot
Isolated chromatin from untreated HUVECs and treated with TGFb2.
1:1000 dilution in 5% skim milk in TBS-t overnight at 4 degrees
5% skim milk in TBS-t for 1 hour at room temperature
1:10,000 in 5% skim milk in TBS-t for 2 hours at room temperature (anti-rabbit Thermo Scientific Cat#31460)
NA
Enhanced chemiluminescence
Band detected at 15kDa which showed decreased expression in HUVECs after treatment with TGFb2. Exposure time of 10 seconds with film.
None
This antibody detected a single band, very clean Western
Would definitely recommend this antibody