Vectashield with DAPI does exactly what you want, no fuss

January 12, 2015

Florida State University
Biological Science
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Vector Laboratories

Product Name:

Vectashield with DAPI

Catalog Number:

H-1200

You drip a bit of this onto your tissue on the slide, coverslip, and you will have strong DAPI staining with low aberrant staining. Works best with a brightfield fluorescent microscope and not confocal microscopy. Cheap and keeps in the refrigerator for some time.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

Brain tissue, fixed

Protocol Overview

Perform immunohistochemical staining as normal. Once protocol is complete, mount tissue onto slides. When tissue has dried and affixed to slide, apply a few drops of the Vectashield with DAPI onto your tissue. Coverslip. View using a fluorescence-compatible microscope that can 'view' within the range of DAPI.

Tips

It really is quite simple to use.

Results Summary

Product gave strong staining and low aberrant staining. DAPI signal was strong and easily visible.

Additional Notes

None

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Summary

The Good

Easy to use, effective

The Bad

May not be as cheap as what you are doing now, may give too strong a signal for confocal microscopy

The Bottom Line

If you want an easy-to-use DAPI stain without buying DAPI separately, this product performs well.

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