Nice LC3B antibody for tougher protein

January 20, 2015

Pharmaceutical Sciences
North Dakota State University
Research Assistant

Overall

Quality of Results

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Company:

Cell Signaling Technology

Product Name:

LC3B Antibody

Catalog Number:

2775

Browse Similar Products: LC3B Western blot Antibodies

This antibody was used in a study that investigated the role of LC3-B in autophagy. Several LC3-B antibodies were tested in this study for various applications; the antibody reviewed here (2775) gave the best results in Western blot staining of LC3-B in pancreatic cancer cells.

Experimental Design and Results Summary

Applications

Western blot

Sample

Pancreatic cancer cells

Primary Incubation

1:1000 overnight at 4 C

Blocking Agent

5% Milk TBST

Secondary Incubation

1:10000 at room temperature for 1 hour

Tertiary Incubation

NA

Detection

HRP chemiluminescence

Results Summary

This antibody worked excellently in Western blot analysis of cells overexpressing LC3-B. Only minimal background staining compared to isotype control could be detected.

Additional Notes

None

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Summary

The Good

Highly specific, low background

The Bad

Troubleshooting needed to achieve perfect blot results

The Bottom Line

This is a very good antibody to detect LC3-B in cancer cells that overexpress autophagy related genes. The minimal background staining is tolerable when comparing over-expressing cells.

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