Good invariant chain intracellular staining

July 17, 2015

BlueskyReddit
Immunology
University
Graduate Student

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Company:

BD Biosciences

Product Name:

FITC Rat Anti-Mouse CD74

Catalog Number:

555318

Browse Similar Products: CD74 Antibodies

We used this antibody to investigate the functionality of the MHC II antigen presentation pathway in murine cells using flow cytometry. This antibody stains well intracellularly using BD Biosciences' cytofix/cytoperm kit

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary murine DC and B cells

Primary Incubation

1:100 for 30 minutes at 4 degrees C

Blocking Agent

2.4G2 Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Canto II

Results Summary

Good intracellular staining using BD Biosciences' cytofix/cytoperm kit. Some extracellular staining of unclear significance.

Additional Notes

None

Image Gallery

Summary

The Good

Stains well

The Bad

Unclear what the extracellular staining represents as this is supposed to be an intracellular epitope

The Bottom Line

Good invariant chain staining.

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