Excellent Antibody to Detect SirT1 in Human Cell Lines

July 23, 2015

Physiology and Biochemistry
Junagarh Agricultural Univerisity
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

SirT1 (D739) Antibody

Catalog Number:

2493

Info: View Product View Product Specs

Browse Similar Products: SirT1 Antibodies

The aim of the study was to test the time course effect of VEGFA on SirT1 expression in human endothelial cells. We found Cell Signaling Technology's SirT1 (D739) Antibody on Biocompare.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human endothelial cells

Primary Incubation

1:1000 (5% skimmed milk in TBST), Overnight at 4 degrees Celsius

Blocking Agent

5% Skimmed milk in TBST, 2h at RT

Secondary Incubation

1:4000 (5% Skimmed milk in TBST), 1h at RT

Tertiary Incubation

NA

Detection

ECL

Results Summary

There was no effect of VEGFB on sirT1 expression in human retinal endothelial cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Got only the SirT1 band. No non-specific band was detected

The Bad

None

The Bottom Line

Excellent antibody for immunoblotting in human cell lines

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