This Antibody Can be Used for Immunohistochemistry and Western Blot Study

pathology
upmc
research associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

HA-Tag

Catalog Number:

2367S

I do cloning for animal experiments to use as liver cancer models and thus use a variety of molecular biology reagents including antibodies, plasmids, and RNA kits. For this antibody, just follow the manual's instruction, 1:200 dilution for IHC staining, 1:1000 used for WB study, and then do the experiment accordance with the IHC or Western blot protocol.

Experimental Design and Results Summary

Applications

IHC and WB

Sample

Paraffin block slides or protein lysis

Primary Incubation

Just follow the general protocol for IHC and Western blot and there is no additional requirement.

Blocking Agent

Standard protocol

Secondary Incubation

Standard protocol

Tertiary Incubation

NA

Detection

ECL

Results Summary

It's a reliable antibody for HA-tag marked gene study.

Additional Notes

None

Related Categories

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Summary

The Good

Works well

The Bad

None

The Bottom Line

Works well

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