Great anti-Ubiquitin antibody for total cell lysate

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology Inc.

Product Name:

Ub (P4D1)

Catalog Number:

sc-8017

Info: View Product View Product Specs

Browse Similar Products: P4D1 Antibodies

We analyze sample of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according to their protein content. This antibody recognizes the endogenous protein from Arabidopsis; it works very well for plant extracts. Ubiquitin and ubiquitinated proteins can be easily detected using this antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Arabidopsis seedlings or leaves and recombinant protein

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1:2500

Tertiary Incubation

NA

Detection

ECL

Results Summary

Ubiquitin and ubiquitinated proteins both recombinant and endogenous can be easily detected using this antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good clear signal and reproducibility

The Bad

Low concentration

The Bottom Line

Great anti-Ubiquitin antibody

Share your experience with other scientists. Write a Review! »

Join the discussion