The DeNovix DS-11 Spectrophotometer

Our group does a lot of cloning using restriction enzymes. Typically, we have small volumes of the materials along with low quantities after purification of the digested vector and insert by agarose electrophoresis. However, it is important to know the concentration of insert and vector DNA for successful ligation of a DNA insert into a cloning or expression vector because both the absolute DNA concentration and the ratio between vector and insert DNA affect the efficiency of the ligation reaction. The DeNovix DS-11 is a compact spectrophotometer that ensures precise nucleic acid measurements for samples as little as 0.5 µL. The instrument offers very simple and fast operation: pipet a drop of sample, measure (just 4 seconds per measurement) and wipe off, which is especially handy when you are dealing with multiple samples.