Antibody Used for Western Blot

upmc
pathology
research associate

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Company:

Life Technologies

Product Name:

v5 tag

Catalog Number:

1408864

I do cloning for animal experiments to use as liver cancer models and thus use a vareity of molecular biology reagents including antibodies, plasmids, and RNA kits. I always try to get the right reagent for the lowest price. We performed many Western blots using this antibody. It is a mouse Monoclonal Antibody that detects recombinant proteins containing V5 tag.

Experimental Design and Results Summary

Application

Western blot

Starting Material

Protein lysis

Protocol Overview

General WB protocol, no additional requirement

Tips

None

Results Summary

This is a reliable antibody for Western blot for V5 tag, the dilution is from 1:1000 - 1:5000 and it can also be used for IF and ELISA experiments

Additional Notes

None

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Summary

The Good

Works well

The Bad

None

The Bottom Line

The dilution could be brought to 1:10000 dilution for a v5 tag gene over-expressed experiment.

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