Life Technologies
v5 tag
1408864
I do cloning for animal experiments to use as liver cancer models and thus use a vareity of molecular biology reagents including antibodies, plasmids, and RNA kits. I always try to get the right reagent for the lowest price. We performed many Western blots using this antibody. It is a mouse Monoclonal Antibody that detects recombinant proteins containing V5 tag.
Western blot
Protein lysis
General WB protocol, no additional requirement
None
This is a reliable antibody for Western blot for V5 tag, the dilution is from 1:1000 - 1:5000 and it can also be used for IF and ELISA experiments
Works well
The dilution could be brought to 1:10000 dilution for a v5 tag gene over-expressed experiment.