Extremely reliable anti-His antibody

July 15, 2015

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

GE Healthcare

Product Name:

Mouse Anti-His Antibody

Catalog Number:

27-4710-01

Browse Similar Products: His Antibodies

We analyzed samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according to their protein content. His-tagged proteins in Arabidopsis tissue extracts and in samples containing recombinant protein can be easily detected using this antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Arabidopsis seedlings or leaves and recombinant protein

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

NA

Detection

ECL

Results Summary

HA-tagged proteins can be easily detected using this antibody, regardless whether recombinantly expressed in bacteria, or transiently or stably transformed and expressed in plants.

Additional Notes

None

Image Gallery

Summary

The Good

Very good, clear signal and reproducibility

The Bad

High price, relatively high unit price

The Bottom Line

Extremely reliable anti-His antibody

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