Cell Signaling
Acetyl-Stat3 (Lys685) Antibody
2523
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The aim of our research was to test the time course effect of VEGFB on STAT3 (Lys685) acetylation in human endothelial cells by immunoblotting. Cell Signaling Technology's antibody is good for immunoblotting so we selected the above antibody.
Western Blot
Human endothelial cells
1:1000 (5% BSA in TBST), Overnight at 4 degrees celsius
5% Skimmed milk in TBST, 2h at RT
1:4000 (5% Skimmed milk in TBST), 2h at RT
NA
ECL
VEGFB does not have any effect on STAT3 acetylation
No non-specific background detected
None
Best antibody