Thermo Fisher Scientific Pierce
BCA Protein Assay Reagent Kit
23225
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Protein estimation is routine work that is generally performed prior to western blotting, SDS-PAGE etc. I use this kit for estimating the total proteins in whole cell lysates for further quantitative estimation of several proteins in different samples. This kit is better than standard nanodrop quantification because of high reproducibility, no errors and background color.
Protein estimation
Whole cell lysates and standards
Product A, B and C were mixed together in the ratio of 25:24:1 to make a working solution. 2 microlitres of standards were mixed with 300 microlitres of working solution. The samples were incubated at 60 degrees celcius put into a waterbath for 45 minutes, followed by reading absorbance at 592 nm.
Minimize pipetting errors by using different tips for each time pipetting.
All standards provided with this kit showed a linear graph against their absorbance. Hence, protein concentration is directly proportional to their absorbance. Using this graph, we can easily calculate the protein concentration of unknown samples.
None
Quick and reliable quantification
One needs to be really careful with pipetting in order to avoid errors.
Excellent kit for protein quantification
Product Name: BCA Protein Assay KitSupplier Page