B220 PECy7 Antibody Review

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

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Company:

BioLegend

Product Name:

B220/CD45 PECy7 Antibody

Catalog Number:

103221

This antibody was used to identify and sort out specific B cell populations in latently infected mouse peritoneum.

Experimental Design and Results Summary

Applications

Cell Isolation/Separation

Sample

Peritoneal Exudate Cells

Primary Incubation

1:100 15 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:400 15 minutes

Tertiary Incubation

NA

Detection

Facs Aria

Results Summary

B Cells are correctly identified in order to sort out B cells from non B cells in mouse peritoneum.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable for sorting, Good Signal

The Bad

None

The Bottom Line

Good antibody to identify a broad B cell population.

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