Miltenyi Biotec
CD14 Microbeads
130-050-201
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With this product we achieved successful isolation of monocytes from human PBMCs with >90% purity of CD14+ monocytes and functionally active cells (phagocytosis and cytokine release)
Flow Cytometry
PBMCs
Isolate monocytes, add myelin debris, measure myelin debris, phagocytosis by flow cytometry
Some cells may be activated by CD14 microbeads
Great results for myelin debris phagocytosis assays and ELISA assays from monocyte supernatants
None
Quick and easy protocol, optimized to use less beads and still obtain a pure monocyte population, reasonable price.
Potential activation
Great fast and easy way to obtain high monocyte purity!