Qiagen
RT2 First Strand Kit
330404
I used this kit to screen positive colonies of transformants of ESAT6. It has a genomic DNA removal step which helps to avoid genomic DNA contamination in qPCR/RT-PCR. I would recommend this kit to my colleagues for quick and efficient cDNA synthesis.
Reverse transcriptase PCR
Total RNA
I used 1 microgram of RNA to synthesize cDNA from different colonies.
None
I got most of the positive colonies from my screening.
In this image, I am showing amplification of the amplicillin resistance gene, that was used an internal loading control.
Quick, efficient, and reproducible
In conclusion, it's a good kit.