Abcam
Anti-EpCAM antibody [G8.8]
ab92382
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To analyze the activation of mouse intestinal macrophages after experimental treatment, mice were gavaged with reagent and intestinal tissue was collected after 24 hours. Intestinal tissue was snap frozen and cryosections were used for staining the intestinal epithelial cells and/or macrophages. We found intestinal macrophages (F4/80 +) activation and the EpCAM positive cells showed the structure of the layer of epithelia cells (EpCAM positive cells shown in figure).
Immunofluorescence
Mouse intestinal tissue
Anti-EpCAM antibody (1:100), incubated at RT for 1hr
5% FBS in PBS
Alexa Fluor® 594 anti-rat IgG
NA
Fluorescence microscopy
Results showed that EpCAM positive epithelial cells could be easily found with strong signal in intestine tissue. The structure of the epithelial layer could be identified accordingly.
Do not need high concentration for primary antibody incubation.
Very specific for target cell staining
None
This antibody for IFA can be used to obtain a strong signal in mouse intestine tissue samples. We do not see cross reactions. We have not tested this in other samples like lung tissues.